解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Recent advances toward the characterization of Alzheimer's disease (AD) have permitted the identification of a dozen of genetic risk factors, although many more remain undiscovered. In parallel, works in the field of network biology have shown a strong link between protein connectivity and disease. In this manuscript,...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.114280.110
更新日期:2011-03-01 00:00:00
abstract::Mirtrons are intronic hairpin substrates of the dicing machinery that generate functional microRNAs. In this study, we describe experimental assays that defined the essential requirements for entry of introns into the mirtron pathway. These data informed a bioinformatic screen that effectively identified functional mi...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.113050.110
更新日期:2011-02-01 00:00:00
abstract::The Polycomb group (PcG) and Trithorax group (TrxG) of proteins are required for stable and heritable maintenance of repressed and active gene expression states. Their antagonistic function on gene control, repression for PcG and activity for TrxG, is mediated by binding to chromatin and subsequent epigenetic modifica...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.114348.110
更新日期:2011-02-01 00:00:00
abstract::Advanced prostate cancer can progress to systemic metastatic tumors, which are generally androgen insensitive and ultimately lethal. Here, we report a comprehensive genomic survey for somatic events in systemic metastatic prostate tumors using both high-resolution copy number analysis and targeted mutational survey of...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.107961.110
更新日期:2011-01-01 00:00:00
abstract::LSH, a member of the SNF2 family of chromatin remodeling ATPases encoded by the Hells gene, is essential for normal levels of DNA methylation in the mammalian genome. While the role of LSH in the methylation of repetitive DNA sequences is well characterized, its contribution to the regulation of DNA methylation and th...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.108498.110
更新日期:2011-01-01 00:00:00
abstract::Transcriptional networks have been shown to evolve very rapidly, prompting questions as to how such changes arise and are tolerated. Recent comparisons of transcriptional networks across species have implicated variations in the cis-acting DNA sequences near genes as the main cause of divergence. What is less clear is...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.111765.110
更新日期:2010-12-01 00:00:00
abstract::We present a powerful application of ultra high-throughput sequencing, SAGE-Seq, for the accurate quantification of normal and neoplastic mammary epithelial cell transcriptomes. We develop data analysis pipelines that allow the mapping of sense and antisense strands of mitochondrial and RefSeq genes, the normalization...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.108217.110
更新日期:2010-12-01 00:00:00
abstract::More than 25 loci have been linked to type 1 diabetes (T1D) in the nonobese diabetic (NOD) mouse, but identification of the underlying genes remains challenging. We describe here the positional cloning of a T1D susceptibility locus, Idd11, located on mouse chromosome 4. Sequence analysis of a series of congenic NOD mo...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.101881.109
更新日期:2010-12-01 00:00:00
abstract::The comparison of the chromosome numbers of today's species with common reconstructed paleo-ancestors has led to intense speculation of how chromosomes have been rearranged over time in mammals. However, similar studies in plants with respect to genome evolution as well as molecular mechanisms leading to mosaic synten...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.109744.110
更新日期:2010-11-01 00:00:00
abstract::While copy number variation (CNV) is an active area of research, de novo mutation rates within human populations are not well characterized. By focusing on large (>100 kbp) events, we estimate the rate of de novo CNV formation in humans by analyzing 4394 transmissions from human pedigrees with and without neurocogniti...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.107680.110
更新日期:2010-11-01 00:00:00
abstract::The development of high-throughput sequencing (HTS) technologies has opened the door to novel methods for detecting copy number variants (CNVs) in the human genome. While in the past CNVs have been detected based on array CGH data, recent studies have shown that depth-of-coverage information from HTS technologies can ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.106344.110
更新日期:2010-11-01 00:00:00
abstract::Natural killer (NK) cells are innate lymphocytes important for early host defense against infectious pathogens and surveillance against malignant transformation. Resting murine NK cells regulate the translation of effector molecule mRNAs (e.g., granzyme B, GzmB) through unclear molecular mechanisms. MicroRNAs (miRNAs)...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.107995.110
更新日期:2010-11-01 00:00:00
abstract::Despite the availability of dozens of animal genome sequences, two key questions remain unanswered: First, what fraction of any species' genome confers biological function, and second, are apparent differences in organismal complexity reflected in an objective measure of genomic complexity? Here, we address both quest...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.108795.110
更新日期:2010-10-01 00:00:00
abstract::The expression of most genes is regulated by multiple transcription factors. The interactions between transcription factors produce complex patterns of gene expression that are not always obvious from the arrangement of cis-regulatory elements in a promoter. One critical element of promoters is the TATA box, the docki...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.106732.110
更新日期:2010-10-01 00:00:00
abstract::Initially thought to play a restricted role in calcium homeostasis, the pleiotropic actions of vitamin D in biology and their clinical significance are only now becoming apparent. However, the mode of action of vitamin D, through its cognate nuclear vitamin D receptor (VDR), and its contribution to diverse disorders, ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.107920.110
更新日期:2010-10-01 00:00:00
abstract::Animal microRNA sequences are subject to 3' nucleotide addition. Through detailed analysis of deep-sequenced short RNA data sets, we show adenylation and uridylation of miRNA is globally present and conserved across Drosophila and vertebrates. To better understand 3' adenylation function, we deep-sequenced RNA after k...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.106054.110
更新日期:2010-10-01 00:00:00
abstract::Double minutes (dmin) and homogeneously staining regions (hsr) are the cytogenetic hallmarks of genomic amplification in cancer. Different mechanisms have been proposed to explain their genesis. Recently, our group showed that the MYC-containing dmin in leukemia cases arise by excision and amplification (episome model...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.106252.110
更新日期:2010-09-01 00:00:00
abstract::Chronic neuropathic pain is affected by specifics of the precipitating neural pathology, psychosocial factors, and by genetic predisposition. Little is known about the identity of predisposing genes. Using an integrative approach, we discovered that CACNG2 significantly affects susceptibility to chronic pain following...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.104976.110
更新日期:2010-09-01 00:00:00
abstract::In diploid mammalian genomes, parental alleles can exhibit different methylation patterns (allele-specific DNA methylation, ASM), which have been documented in a small number of cases except for the imprinted regions and X chromosomes in females. We carried out a chromosome-wide survey of ASM across 16 human pluripote...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.104695.109
更新日期:2010-07-01 00:00:00
abstract::Noncoding RNA (ncRNA) constitutes a significant portion of the mammalian transcriptome. Emerging evidence suggests that it regulates gene expression in cis or trans by modulating the chromatin structure. To uncover the functional role of ncRNA in chromatin organization, we deep sequenced chromatin-associated RNAs (CAR...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.103473.109
更新日期:2010-07-01 00:00:00
abstract::The Drosophila Enhancer of split complex [E(spl)-C] is a remarkable complex of genes many of which are effectors or modulators of Notch signaling. The complex contains different classes of genes including four bearded genes and seven basic helix-loop-helix (bHLH) genes. We examined the evolution of this unusual comple...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.104794.109
更新日期:2010-07-01 00:00:00
abstract::Translocations are known to affect the expression of genes at the breakpoints and, in the case of unbalanced translocations, alter the gene copy number. However, a comprehensive understanding of the functional impact of this class of variation is lacking. Here, we have studied the effect of balanced chromosomal rearra...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.103622.109
更新日期:2010-05-01 00:00:00
abstract::X inactivation equalizes the dosage of gene expression between the sexes, but some genes escape silencing and are thus expressed from both alleles in females. To survey X inactivation and escape in mouse, we performed RNA sequencing in Mus musculus x Mus spretus cells with complete skewing of X inactivation, relying o...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.103200.109
更新日期:2010-05-01 00:00:00
abstract::The densities of transposable elements (TEs) in the human genome display substantial variation both within individual chromosomes and among chromosome types (autosomes and the two sex chromosomes). Finding an explanation for this variability has been challenging, especially in light of genome landscapes unique to the ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.099044.109
更新日期:2010-05-01 00:00:00
abstract::Chromosomal rearrangements and copy number variants (CNVs) play key roles in genome evolution and genetic disease; however, the molecular mechanisms underlying these types of structural genomic variation are not fully understood. The availability of complete genome sequences for two bird species, the chicken and the z...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.103663.109
更新日期:2010-04-01 00:00:00
abstract::Next-generation sequencing technologies have made it possible to sequence targeted regions of the human genome in hundreds of individuals. Deep sequencing represents a powerful approach for the discovery of the complete spectrum of DNA sequence variants in functionally important genomic intervals. Current methods for ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.100040.109
更新日期:2010-04-01 00:00:00
abstract::Population genetics has evolved from a theory-driven field with little empirical data into a data-driven discipline in which genome-scale data sets test the limits of available models and computational analysis methods. In humans and a few model organisms, analyses of whole-genome sequence polymorphism data are curren...
journal_title:Genome research
pub_type: 杂志文章,评审
doi:10.1101/gr.079509.108
更新日期:2010-03-01 00:00:00
abstract::It is known that sequencing error can bias estimation of evolutionary or population genetic parameters. This problem is more prominent in deep resequencing studies because of their large sample size n, and a higher probability of error at each nucleotide site. We propose a new method based on the composite likelihood ...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.097543.109
更新日期:2010-01-01 00:00:00
abstract::Cancer progression in humans is difficult to infer because we do not routinely sample patients at multiple stages of their disease. However, heterogeneous breast tumors provide a unique opportunity to study human tumor progression because they still contain evidence of early and intermediate subpopulations in the form...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.099622.109
更新日期:2010-01-01 00:00:00
abstract::During meiosis, chromatin undergoes extensive changes to facilitate recombination, homolog pairing, and chromosome segregation. To investigate the relationship between chromatin organization and meiotic processes, we used formaldehyde-assisted isolation of regulatory elements (FAIRE) to map open chromatin during the t...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.096297.109
更新日期:2009-12-01 00:00:00
abstract::The Y centromere sequence of house mouse, Mus musculus, remains unknown despite our otherwise significant knowledge of the genome sequence of this important mammalian model organism. Here, we report the complete molecular characterization of the C57BL/6J chromosome Y centromere, which comprises a highly diverged minor...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.092080.109
更新日期:2009-12-01 00:00:00
abstract::Live-cell imaging allows detailed dynamic cellular phenotyping for cell biology and, in combination with small molecule or drug libraries, for high-content screening. Fully automated analysis of live cell movies has been hampered by the lack of computational approaches that allow tracking and recognition of individual...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.092494.109
更新日期:2009-11-01 00:00:00
abstract::The common genetic variants associated with complex traits typically lie in noncoding DNA and may alter gene regulation in a cell type-specific manner. Consequently, the choice of tissue or cell model in the dissection of disease associations is important. We carried out an expression quantitative trait loci (eQTL) st...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.095224.109
更新日期:2009-11-01 00:00:00
abstract::Coevolution maintains interactions between phenotypic traits through the process of reciprocal natural selection. Detecting molecular coevolution can expose functional interactions between molecules in the cell, generating insights into biological processes, pathways, and the networks of interactions important for cel...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.092452.109
更新日期:2009-10-01 00:00:00
abstract::Pollen, the male gametophyte of flowering plants, represents an ideal biological system to study developmental processes, such as cell polarity, tip growth, and morphogenesis. Upon hydration, the metabolically quiescent pollen rapidly switches to an active state, exhibiting extremely fast growth. This rapid switch req...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.089060.108
更新日期:2009-10-01 00:00:00
abstract::We describe a new method, Tag-seq, which employs ultra high-throughput sequencing of 21 base pair cDNA tags for sensitive and cost-effective gene expression profiling. We compared Tag-seq data to LongSAGE data and observed improved representation of several classes of rare transcripts, including transcription factors,...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.094482.109
更新日期:2009-10-01 00:00:00
abstract::Interindividual variability in response to chemicals and drugs is a common regulatory concern. It is assumed that xenobiotic-induced adverse reactions have a strong genetic basis, but many mechanism-based investigations have not been successful in identifying susceptible individuals. While recent advances in pharmacog...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.090241.108
更新日期:2009-09-01 00:00:00
abstract::ClinSeq is a pilot project to investigate the use of whole-genome sequencing as a tool for clinical research. By piloting the acquisition of large amounts of DNA sequence data from individual human subjects, we are fostering the development of hypothesis-generating approaches for performing research in genomic medicin...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.092841.109
更新日期:2009-09-01 00:00:00
abstract::We present a database of copy number variations (CNVs) detected in 2026 disease-free individuals, using high-density, SNP-based oligonucleotide microarrays. This large cohort, comprised mainly of Caucasians (65.2%) and African-Americans (34.2%), was analyzed for CNVs in a single study using a uniform array platform an...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.083501.108
更新日期:2009-09-01 00:00:00
abstract::An open question in bacterial genomics is the role that adaptive evolution of the core genome plays in diversification and adaptation of bacterial species, and how this might differ between groups of bacteria occupying different environmental circumstances. The genus Campylobacter encompasses several important human a...
journal_title:Genome research
pub_type: 杂志文章
doi:10.1101/gr.089250.108
更新日期:2009-07-01 00:00:00